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. 2013 Oct 17;8(10):e76547. doi: 10.1371/journal.pone.0076547

Figure 8. Immunocytochemistry analysis on the hESC expression of OCT4 and SSEA3 after being cultured in the media supplemented with Mg ion dosages.

Figure 8

All H9-OCT4 hESCs cultured in the media supplemented with different dosages of Mg ions expressed markers for OCT4 and SSEA3. The images for 0.4 mM media condition were not shown as they were very similar to the control. Nuclear localization of OCT4 is shown in green (left column) and SSEA3 expression on the cell surface is shown in red (middle column). The right column shows merged images of the OCT4, SSEA3, and DAPI (blue, cell nucleus). Scale bars = 100 µm.