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. 2013 Oct 17;8(10):e77469. doi: 10.1371/journal.pone.0077469

Figure 5. DNA damage in cells exposed to either gDNA or gDNAOX at final concentration 50 ng/mL for 30 min and 2 hours.

Figure 5

А – comet assay in alkaline conditions [1]. - Digital photography of the nuclei with varying degree of DNA damage [2,3]; - cumulative histograms for tail moment and percentage of DNA within tails. The reliability of differences with the control in the obtained distributions was analyzed by means of Kolmogorov–Smirnov statistics (the table shows the values of D and α).

B - dsDNA breaks in cells exposed to gDNAOX (50ng/mL, 1 hour).Cells were processed for immunofluorescence staining with anti γH2AX antibody (x40) [1].- Three detected types of nuclei are denoted by numbers: 1- nucleus with multiple dsDNA breaks, 2- nucleus with a few dsDNA breaks, 3- nucleus with intact DNA [2]. - Example of a micronucleus with dsDNA breaks.

С – FACS analysis of γ-foci A: there main fractions of the cells as evident in gating areas R1, R2, R3 [1], the distribution of γH2AX fluorescence intensities [2], relative proportions of cells within gating areas R1-R3 [3]. *p < 0.05 against control group of cells, non-parametric U-test.