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. 2013 Aug 28;288(42):30009–30018. doi: 10.1074/jbc.M112.443093

FIGURE 2.

FIGURE 2.

The disruption of Bim alleviated spontaneous hepatocyte apoptosis in the absence of Mcl-1. The offspring from the mating of bim+−mcl-1flox/floxalb-cre mice with bim+/−mcl-1flox/flox mice were examined at 6 weeks of age. Mcl-1+/+ and Mcl-1−/−, mcl-1flox/flox and mcl-1flox/floxalb-cre, respectively. A, Western blot analysis of whole liver lysates for the expression of BimEL, Bid, Bcl-xL, Mcl-1, Bak, Bax, cleaved caspase-3, cleaved caspse-7, and β-actin. B, representative images for liver histology stained with hematoxylin-eosin (HE), TUNEL, and cleaved caspase-3 (original magnification, ×100). C, TUNEL-positive cell ratio; n = 3–6 mice/group; *, p < 0.05 versus all. D, cleaved caspase-3-positive cell ratio; n = 3 mice/group; *, p < 0.05 versus all. E, serum caspase-3/7 activity; n = 9–15 mice/group; *, p < 0.05 versus all. F, serum ALT levels; n = 9–15 mice/group; *, p < 0.05 versus all. Error bars, S.D. RLU, relative light units; I/U, international units.