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. 2013 Aug 28;288(42):30009–30018. doi: 10.1074/jbc.M112.443093

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© 2013 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 3. — The disruption of Bim and Bid prevented spontaneous hepatocyte apoptosis in the absence of Bcl-xL. The offspring from the mating of bim^+/−bid^−/−bcl-x^flox/floxalb-cre mice with bim^+/−bid^−/−bcl-x^flox/flox mice were examined at 6 weeks of age. Bcl-xL^+/+ and Bcl-xL^−/−, bcl-x^flox/flox and bcl-x^flox/floxalb-cre, respectively. A, Western blot analysis of whole liver lysates for the expression of Bim_EL, Bid, Bcl-xL, Mcl-1, Bak, Bax, and β-actin. B, representative images of liver histology stained with hematoxylin-eosin (HE) and TUNEL (original magnifications, ×100 (large panels) and ×400 (insets)). Black arrows indicate apoptotic bodies. C, TUNEL-positive cell ratio; more than 5 mice/group; *, p < 0.05 versus all. D, serum caspase-3/7 activity; more than 6 mice/group; *, p < 0.05 versus all. E, serum ALT levels; more than 6 mice/group; *, p < 0.05 versus all. Error bars, S.D. RLU, relative light units; I/U, international units.