FIGURE 1.
eIF3m is essential for mouse embryonic development. A, expression patterns of eIF3m in different mouse tissues. Cell lysates were prepared from an 8-week-old male mouse. GAPDH served as the loading control. B, strategy for eIF3m knock-out. Exons 2–5 of eIF3m, followed by a neomycin resistance cassette (neor), were flanked with two loxP sites (triangles). Exons 2–5 and neor were then deleted by Cre/loxP recombination, producing the eIF3m−/− (knock-out) allele. C, representative genotyping results obtained by PCR using tail DNA from one wild-type (eIF3m+/+) and three eIF3mflox/+ mice. D and E, genotyping of mice or embryos produced by crossing eIF3m+/− mice. F, genotyping of postnatal mice produced by crosses between eIF3mflox/flox and eIF3m+/− mice. G, representative images of eIF3mflox/+ and eIF3mflox/− embryos at E9.5.