FIGURE 8.

NET blockade is required for chronic immobilization stress-induced bone loss. A–D, CIS does not induce bone loss in 3-month-old WT mice. Micro-CT analyses of femoral (A and B) and vertebral (C and D) trabecular bones in males and females. E, CIS induces bone loss in male WT mice only when NE reuptake is inhibited. F, concurrent CIS and NET blockade reduces the number of bone marrow osteoprogenitors (assessed by the number of Cfu-AP colonies, right panel) compared with the control or CIS groups, following 14 days of in vitro differentiation in osteogenic condition. Values are given as mean ± S.E., *, p < 0.05 versus control; #, p < 0.05 versus CIS, n ≥ 8.