FIGURE 6.

Effect of sAPPα inhibition on colony formation by pancreatic cancer cells. A, 5,000 CD18 cells were seeded in soft agar and treated with 1 μm batimastat, 100 ng/ml gemcitabine or combination of both drugs (left panel in A) to study their effect on the ability of cells to form colonies over a period of 2 weeks. Soft agar assay performed with CD18 cells using 5 μm batimastat, 100 ng/ml gemcitabine or a combination of both drugs (right panel in A). Similarly, 5,000 MiaPaCa2 (B) or Panc1 (C) cells were seeded in soft agar and treated with 10 μm batimastat, 100 ng/ml gemcitabine, or a combination of the drugs to study their effect on colony formation. Treatment with each dose was performed in triplicate, and each experiment was performed three times.