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. 2013 Sep 11;288(42):30139–30150. doi: 10.1074/jbc.M113.493478

FIGURE 1.

FIGURE 1.

TEN1 depletion in human cancer cells. A, RT-qPCR of TEN1 mRNA in various cell lines. Levels are relative to shNT for each cell type with normalization to GAPDH (n = 4 for shTEN1–5; n = 3 for HCT116 and HeLa shTEN1#1; n = 2 for HT1080, HeLa shTEN1#1+#2, and shTEN1–5R; means ± S.D.). Note discontinuous y axis. p values are indicated. B, Western blot showing TEN1 (13 kDa) and STN1 (42 kDa) levels in two HCT116 pools: HeLa1.2.11 shSTN1–7, shTEN1–5, and TEN1–5R clones and HT1080 shSTN1 and shTEN1 pools. Loading control is α-actinin (100 kDa). The total amount of cell lysate is indicated at the top. The asterisk indicates a nonspecific band that cross-reacts with the STN1 antibody, and the arrow indicates STN1. C, representative growth curves for HeLa clones. n = 2 experiments. The cells were counted in triplicate at each time point.