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. 2013 Sep 11;288(42):30139–30150. doi: 10.1074/jbc.M113.493478

FIGURE 2.

FIGURE 2.

TEN1 is needed for telomere integrity. A, telomere FISH on metaphase spreads of shNT, shTEN1–5, or shTEN1–5R cells showing examples of MTS (white arrows) or SFE (red arrows). Green, FITC-telomere probe; blue, DAPI. Chromosomes in insets are enlarged 2-fold. Scale bars are indicated. B, quantification of MTS and SFE (means ± S.D., n = 3). C, γ-H2AX staining combined with telomere FISH on metaphase spreads from shNT (left) or shTEN1–5 (right) cells. White arrows, SFE; yellow arrows, TIFs; green, γ-H2AX; red, Cy3-(C3TA2)3 probe; blue, DAPI. Chromosomes in insets are enlarged 5-fold. Right panel, positive control showing γ-H2AX staining of nuclei after treatment of shNT cells with 2 mm HU for 24 h. Scale bars are indicated. D, chromosome orientation FISH on metaphase spreads showing examples of chromosomes with and without T-SCE. Red, Cy3-(C3TA2)3 probe; green, FITC-(T2AG3)3 probe; blue, DAPI. Scale bars are indicated. E, quantification of T-SCE (means ± S.D., n = 3).