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The effect of TEN1 depletion on telomere length varies with cell type and level of knockdown. A–C, telomere lengths in HeLa1.2.11 (A), HCT116 (B), or HT1080 (C) cells at various times after introduction of shRNA. Day 0 corresponds to final day of lentivirus transfection. Telomeric restriction fragments were visualized by in-gel hybridization with (TA2C3)4 probe. mRNA depletion and mean telomere length for individual time points are shown below each gel.
