FIGURE 1.

Design and expression of FV B-domain fragments. A, structural organization of FV. The BR (Lys⁹⁶³–Lys¹⁰⁰⁸) is indicated in blue, and the AR (Thr¹⁴⁹²–Asn¹⁵³⁸) is indicated in red. Amino acid sequences of the BR and AR used to design B-domain fragments are shown below. B, SDS-PAGE analysis of purified B-domain fragments. Purified proteins (2 μg/lane) were resolved on 4–12% gels under reducing conditions and stained with Coomassie Brilliant Blue. First lane, the BR peptide; second lane, the acetylated BR peptide (Ac-BR); third lane, the BR+AR peptide. The apparent molecular weights of the protein standards are indicated.