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Dose-dependent vesiculation and tubulation of TREs by EHD proteins. 0.1, 1, and 2 μg of either GST alone, GST-EHD1, GST-EHD2, GST-EHD3, or GST-EHD4 was used in the semipermeabilized vesiculation assay. MICAL-L1-containing TREs per field (A) or total area of MICAL-L1-containing TREs were analyzed (B) by ImageJ and plotted.
