FIGURE 4.

Comparison of the ScS (sulfate) and ScP (perchlorate) aggregates by SDD-AGE and sonication. A, sonication in the same conditions generates a large fraction of monomeric/dimeric protein in the sulfate-produced sample as compared with the perchlorate-produced sample. The images indicate higher frangibility of the sulfate-produced amyloids. B, amyloids generated in sulfate can enter the gel even without sonication, indicating the higher abundance of shorter fibers as compared with amyloids produced in perchlorate that cannot enter the gel in these conditions. After sonication, a larger amount of the sulfate-produced amyloid enters the gel as compared with perchlorate-produced amyloid. This confirms that the fibers formed in sulfate were more frangible than those formed in perchlorate. The gel in panel B was run for a longer time than the gel in panel A to resolve the upper limit of the polymer bands.