T3 induced mRNA expression of PCK1 and G6PC in human hepatic cells ectopically expressing TRβ1 (TRβ1-HepG2) and in primary mouse hepatocytes.
A and B, cells were treated with or without T3 (at 0.1 μm concentration) for 24 h. Total mRNA was collected, and RT-qPCR analysis was done as indicated under “Experimental Procedures.” The figure shows mRNA expression of PCK1 and G6PC in HepG2 cells ectopically expressed TRβ1 (A) and primary mouse hepatocytes (B). C, T3 significantly increased PCK1 and G6PC mRNA expression in a time-dependent manner (at 0.1 μm concentration), as well as dose-dependent manner (for 24 h) in TRβ1-HepG2 cells. Cells were treated with or without T3 at the indicated time points or concentrations and maintained in normal T3-depleted medium as indicated under “Experimental Procedures.” β-Actin was used as normalization control (n = 3; *, p < 0.05). Error bars represent mean ± S.D.