FIGURE 5.

The D340A and D1042A mutant proteins fail to localize to the plasma membrane. A, we determined resistance of the mutants to clo in liquid culture with isogenic WT and Δpdr5 strains, as described in Fig. 3. ■, WT; ▴ and solid line, Δpdr5; * and dashed line, D340A; × and dotted line, D1042A. B, Western blotting of PM vesicles from D340A and D1042A was performed as described under “Experimental Procedures.” We used 10 μg of PM vesicle protein made from double-copy strains in each sample and overexposed the blot. C, Western blot of the PM vesicle proteins (10 μg) made from the remaining strains used in this study. The conditions are described in B.