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Analysis of D1042N and D1042E mutations. We determined the relative resistance of the Asp-1042 mutants to clo (A), cyh (B), imazalil (C), cyproconazole (D), and tebuconazole (E) in liquid culture as described under “Experimental Procedures.” In these experiments, n = 3. In the panels with the killing curves, ■ and green line, WT; ▴ and red line, Δpdr5; □ and violet line, D1042E; ● and blue line, D1042N.
