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Analysis of R6G transport in whole cells demonstrates that the D1042N mutant is severely impaired. A, R6G efflux in whole cells was performed as described in the legend to Fig. 3 and under “Experimental Procedures.” ■, WT; ▴, ΔPdr5; ●, D1042N; □, D1042E. In these experiments, n = 2. B, histogram plots for cells showing the retained fluorescence after 30 min in 0.05 m Hepes, 1 mm glucose buffer. The positions of the major peaks for WT, ΔPdr5, D1042E, and D1042N are indicated.
