FIGURE 8.

The behavior of single- versus double-copy strains. A and B, we determined the relative resistance of single- and double-copy strains in liquid culture as described under “Experimental Procedures” with clo (A) and cyh (B). The single-copy data are the same as those found in Fig. 6. For each allele, the solid line is the single-copy strain, the dashed line is the double copy. In the curves, ■ and green line, WT; □ and violet line, D1042E; ● and blue line, D1042N. C, we determined R6G efflux with the single- and double-copy strains, as described under “Experimental Procedures.” The symbols and lines are the same as those used in A and B for WT and D1042N. In these experiments, n = 4.