FIGURE 9.

ATPase activity of D1042N and D1042E: D1042N has nearly WT catalytic capability. ATPase activity of PM vesicles purified from single-copy (A) and double-copy (B) D1042N strains and single (C) and double-copy (D) D1042E strains was determined as previously described (20), with the reaction conditions described in the legend to Fig. 4. Representative plots are shown for each strain (see Table 2). ■, WT; □, D1042E; ●, D1042N. We monitored the effect of adding a WT Pdr5 gene on plasmid pSS607 to the D1042N mutant on resistance to clo (E) in liquid culture following transformation and strain verification. WT (■), D1042N (●), and D1042N + pSS607 (♦) were tested as described in the legend to Fig. 3.