TABLE 1.
Yeast strains
All strains are isogenic to R-1 (14).
| Strain | Genotype |
|---|---|
| R-1 | MATα, PDR1–3, Δpdr5::KANMX4,ura3, yor1, snq2, pdr10, ycf1, pdr11, pdr3 |
| JG2015 | Isogenic to R-1; contains pSS607 integrated at the PDR5 gene as described (14) |
| JG2004 | Isogenic to R-1; contains two WT copies of PDR5 as described previously (14) |
| JG2052 | R-1 + C199A |
| JG2053 | R-1 + E1013A |
| JG2054 | R-1 + G312A |
| JG2064 | Double-copy G312A strain |
| JG2066 | Double-copy E1013A |
| JG2097 | D1042A (5-FOA derivative; see Ref. (9) for preparation of 5-FOA ura3 stocks) |
| JG2098 | D1042A double-copy strain |
| JG2108 | D340A 5-FOA derivative |
| JG2109 | D340A double-copy strain |
| JG2120 | D1042E 5-FOA derivative |
| JG2121 | D1042N 5-FOA derivative |
| JG2122 | D1042E,E1013A 5-FOA derivative |
| JG2126 | D1042E double-copy strain |
| JG2127 | D1042N double-copy strain |
| JG2131 | D1042E,E1013A double-copy |