TABLE 2.
Substitution | Copy no. | Vmax | Km | Figure |
---|---|---|---|---|
nmol Pi/min/mg of protein | mm | |||
WT | 1 | 163.4a | 0.76 | Fig. 9A |
1 | 197.6a | 1.25 | Fig. 9C | |
2 | 221.4 ± 18.81 | 0.80 ± 0.22 | Fig. 4 | |
2 | 287.6 ± 58.07 | 0.83 ± 0.33 | Fig. 9 (B and D) | |
2 | 331.0 ± 44.65 | 0.75 ± 0.27 | ||
E1013A | 2 | 68.42 ± 10.26 | 1.00 ± 0.37 | Fig. 4 |
2 | 99.15 ± 10.74 | 0.29 ± 0.11 | ||
C199A | 2 | 62.13 ± 4.836 | 0.25 ± 0.09 | Fig. 4 |
D1042E | 1 | 67.99 + 3.083 | 0.43 ± 0.07 | Fig. 9C |
2 | 132.4 ± 6.176 | 0.59 ± 0.09 | Fig. 9D | |
D1042N | 1 | 106.8 ± 19.29 | 1.16 ± 0.50 | Fig. 9A |
2 | 187.9 ± 18.71 | 0.60 ± 0.19 | Fig. 9B |
a Only one determination of WT activity was made in this experiment.