TABLE 2.

ATPase activity of mutant enzymes

Substitution	Copy no.	Vmax	Km	Figure
		nmol Pi/min/mg of protein	mm
WT	1	163.4a	0.76	Fig. 9A
	1	197.6a	1.25	Fig. 9C
	2	221.4 ± 18.81	0.80 ± 0.22	Fig. 4
	2	287.6 ± 58.07	0.83 ± 0.33	Fig. 9 (B and D)
	2	331.0 ± 44.65	0.75 ± 0.27
E1013A	2	68.42 ± 10.26	1.00 ± 0.37	Fig. 4
	2	99.15 ± 10.74	0.29 ± 0.11
C199A	2	62.13 ± 4.836	0.25 ± 0.09	Fig. 4
D1042E	1	67.99 + 3.083	0.43 ± 0.07	Fig. 9C
	2	132.4 ± 6.176	0.59 ± 0.09	Fig. 9D
D1042N	1	106.8 ± 19.29	1.16 ± 0.50	Fig. 9A
	2	187.9 ± 18.71	0.60 ± 0.19	Fig. 9B

^a Only one determination of WT activity was made in this experiment.