FIGURE 6.

Intestinal MTP deletion increases the expression of intestinal fatty acid oxidation genes. A, total RNA isolated from the intestine of 12-week-old WT, I-Mttp^−/−, I-Abca1^−/−, and I-DKO (n = 5) male mice fed a chow diet was used to quantify mRNA levels of different genes. Mean ± S.D., ^a, p < 0.05; ^b, p < 0.01, and ^c, p < 0.001 compared with WT. B, in a separate experiment, 12-week-old WT, I-Mttp^−/−, I-Abca1^−/−, and I-DKO male mice (n = 3) were fasted overnight and sacrificed. For fatty acid oxidation, 50-mg aliquots of intestine were incubated with [¹⁴C]oleic acid (0.3 μCi) for 2 h, and the radiolabeled CO₂ was captured and quantified in a scintillation counter. Mean ± S.D. **, p < 0.01 and ***, p < 0.001 compared with WT.