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. 2013 Sep 6;288(42):30473–30484. doi: 10.1074/jbc.M113.489914

FIGURE 6.

FIGURE 6.

Proteolytic activity of B. cenocepacia K56-2 wild-type, ΔatsR, ΔatsRΔcepI, and ΔcepI mutants and complemented mutants at the chromosomal level in different genetic backgrounds. A, proteolysis was tested on D-BHI milk agar plates. The plates shown are representatives of three experiments performed in triplicate. Zones of clearing around the colonies were measured at 48 h of incubation at 37 °C. B, values are the average radius ± S.D. in millimeters of three experiments performed in triplicate. C, anti-His Western blot analysis of the His-tag-purified membrane pellet of AtsR, AtsRΔRD, AtsRD536A, and AtsRH245A in B. cenocepacia. Arrows indicate the positions of full-length AtsR (AtsR, AtsRD536A, and AtsRH245A) and AtsRΔRD. AtsR-FL, full-length AtsR.