FIGURE 8.

Proteolytic activity of B. cenocepacia ΔatsT, ΔatsRΔatsT atsRΔRD⁺, ΔatsRΔcepIΔatsT atsRΔRD⁺, ΔatsRΔatsT atsR_D536A⁺, and ΔatsRΔcepIΔatsT atsR_D536A⁺. A, atsT was deleted from B. cenocepacia K56-2 ΔatsR atsRΔRD⁺, ΔatsRΔcepI atsRΔRD⁺, ΔatsR atsR_D536A⁺, and ΔatsRΔcepI atsR_D536A⁺ backgrounds. Mutants were tested on D-BHI milk agar plates. The plates shown are representatives of three experiments performed in triplicate. Zones of clearing around the colonies were measured at 48 h of incubation at 37 °C. B, atsT was deleted from B. cenocepacia K56-2 ΔcepI background, and the resulting strain was complemented with either atsT or atsT_D208A at the chromosomal level. Mutants were tested on D-BHI milk agar plates. The plates shown are representatives of three experiments performed in triplicate. C, values are the average radius in millimeters of three experiments performed in triplicate.