TABLE 2.
Oligonucleotide primers
NA indicates the absence of a restriction site.
| Primer no. | 5'-3' Primer sequencea | Restriction enzyme |
|---|---|---|
| 2836 | TTTGCTCGAGTTGTTGGCGGTGATGAGAT | XhoI |
| 2839 | TAGGAATTCAGGATCACGCCGTACTTGTC | EcoRI |
| 2840 | TTTTCTCGAGCACATCGTGTCGCACTACAA | XhoI |
| 2844 | TACGTCTAGAATCTTTAGGGCGATCGGGAAC | XbaI |
| 2869 | TTTCATATGTTTATCGTCGTCATCTTTGT | NdeI |
| 4009 | TTTTTCTAGACACCGAGCAACGCTACAC | XbaI |
| 4010 | TTTTCTCGAGCGTGATGGCCTGTTGCAT | XhoI |
| 4011 | TTTTCTCGAGGATATCGTGCTGCATCT | XhoI |
| 4012 | TTTTGAATTCACCTCGTCGTGCTCGATCT | EcoRI |
| 4632 | AAAACATATGACGCGGCGGCGATGGAAGAA | NdeI |
| 4633 | AAAAGCGGCCGCGGCGAGCAGTGTCTCGACGA | NotI |
| 4634 | AAAACATATGCGCACGCGCGACGACCT | NdeI |
| 4736 | AAAAGCGGCCGCTCAGGCGAGCAGTGTCTCGACGA | NotI |
| 4799 | AAAAGCGGCCGCTCATTCGACCGGCAGCGTCAC | NotI |
| 4800 | AAAACATATGGCGCTGGTGGTCGACGAC | NdeI |
| 4880 | GCGTTCCTCGGGATGGTCAGCGCCGAACTGCGCACGCCGCTG | NA |
| 4881 | CAGCGGCGTGCGCAGTTCGGCGCTGACCATCCCGAGGAACGC | NA |
| 5108 | AAAACATATGTCCACCACCGAGCAGGCCAA | NdeI |
| 5109 | AAAAGAATTCTCAGTTCGTCGCGGCCGCTG | EcoRI |
| 5798 | TTTTTTTCATATGCAACAGGCCATCACG | NdeI |
| 5799 | TTTTGCGGCCGCCTACTCGCCGAGCAGATG | NotI |
| 5866 | TTTTTCTAGAGCTTTGTTAGCAGCCGGATC | XbaI |
| 5885 | TTGATGGCGAGCGATTCTTC | NA |
| 5886 | CCAGTTCTTCAGCGTGACGA | NA |
| 5959 | CTCGTCGTGCTCGCGCTCGAACTGCCG | NA |
| 5960 | CGGCAGTTCGAGCGCGAGCACGACGA | NA |
| 6997 | GATGGCTACATCCTCGCCTGGATGCTCGGCG | NA |
| 6998 | GTCGCCGAGCATCCAGGCGAGGATGTAGCC | NA |
| 7020 | TTTTTTCATATGTGGAGACCCCATCGAGATG | NdeI |
| 7021 | TTTTTTCATATGGCATGCCTGCAGGTCGTCAC | NdeI |
a Restriction endonuclease sites incorporated in the oligonucleotide sequences are underlined.