FIGURE 2.

A, WT cortical neuronal cultures at 7 DIV were treated overnight with conditioned media from WT cultures (control (ctrl)), media from WT cultures plus different concentrations of glutamate (2–15 μm as indicated), or media from PS1^−/− cultures. Lysates were then probed by Western blotting with anti-spectrin antibody. The blot shown is representative of four independent experiments with similar results. FL, full-length. B, WT, PS1^+/−, or PS1^−/− neuronal cultures were pretreated for 2 days with dl-TBOA, and media were collected at 7 DIV. The glutamate concentration was then determined using the fluorometric glutamic acid assay kit. Numbers indicate -fold increase of glutamate concentration between connected conditions and represent the average of three independent measurements. Differences were statistically significant. Two-way ANOVA was performed (n = 3).