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. 2013 Sep 1;288(42):30585–30596. doi: 10.1074/jbc.M113.457135

FIGURE 4.

FIGURE 4.

Analyzing the C-terminal domain in ENL protein and its binding to DOT1L. A, alignment of ENL and AF9 C-terminal domain. The three α-helices are indicated, and conserved residues are marked with asterisks. B, binding sensorgrams of immobilized Mocr-DOT1L (826–1095) with ENL (489–544) and ENL (523–559), tested in concentration ranges from 1 to 16 μm and from 5 to 35 μm, respectively. The kon, koff, and KD were calculated by simultaneous nonlinear regression using 1:1 binding model and BIAevaluation 3.1 software. The experimental data are shown in black, whereas the global fit analyses are shown in red. RU, response units.

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