FIGURE 6.
DOT1L 10-mer peptide binds cellular MLL-AF9 fusion protein and disrupts its interaction with DOT1L. A, DOT1L 10-mer peptide disrupts the interaction between DOT1L and MLL-AF9 in cells. FLAG-DOT1L and Myc-CXXC-AF9 were co-transfected in HEK293 cells, and co-immunoprecipitation (IP) was performed. B, pulldown assay using biotin-labeled DOT1L 10-mer peptide. MW, molecular mass.