TABLE 1.
Binding affinities of AF9 and ENL proteins to full-length DOT1L and different constructs of DOT1L immobilized on a CM5 sensor chip and determined by SPR
Recombinant immobilized proteins (residues) | AF9 (497–568) KD ± S.D. | ENL (489–559) KD ± S.D. |
---|---|---|
nm | nm | |
DOT1L (1–1537)a | 33 ± 4 | 206 ± 80 |
DOT1L (826–1095) | 111 ± 10 | 238 ± 72 |
DOT1L (826–958) | 92 ± 8 | 122 ± 16 |
DOT1L (826–937) | 78 ± 15 | 81 ± 6 |
DOT1L (854–1095) | 135 ± 4 | 148 ± 14 |
DOT1L (925–1095) | 1,825 ± 276 | 6,220 ± 113 |
DOT1L (854–925) | 42 ± 2 | 90 ± 12 |
a FLAG-tagged full-length human DOT1L protein.