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. 2013 Sep 1;288(42):30585–30596. doi: 10.1074/jbc.M113.457135

TABLE 1.

Binding affinities of AF9 and ENL proteins to full-length DOT1L and different constructs of DOT1L immobilized on a CM5 sensor chip and determined by SPR

Recombinant immobilized proteins (residues) AF9 (497–568) KD ± S.D. ENL (489–559) KD ± S.D.
nm nm
DOT1L (1–1537)a 33 ± 4 206 ± 80
DOT1L (826–1095) 111 ± 10 238 ± 72
DOT1L (826–958) 92 ± 8 122 ± 16
DOT1L (826–937) 78 ± 15 81 ± 6
DOT1L (854–1095) 135 ± 4 148 ± 14
DOT1L (925–1095) 1,825 ± 276 6,220 ± 113
DOT1L (854–925) 42 ± 2 90 ± 12

a FLAG-tagged full-length human DOT1L protein.

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