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ThiC undergoes steady-state turnover. ThiC (0.55-nmol monomer) was incubated with flavoprotein reductase (0.5 nmol), flavodoxin A (1 nmol), NADPH (0.8 mm), AdoMet (100 μm), AIR (100 μm), and MTAN as indicated (0.1 nmol) at 37 °C. Each data point represents the mean ± S.D. of two replicates from a single experiment. The data were fit to a first-order rate equation, and the 95% confidence intervals of the regression analysis are represented by dotted lines.
