FIGURE 6.
Adenosine is uncompetitive with AdoMet inhibiting ThiC. A, ThiC (0.55-nmol monomer) was preincubated with flavoprotein reductase (0.5 nmol), flavodoxin A (1 nmol), NADPH (0.8 mm), AIR (100 μm), and adenosine (0, 100, 250, or 400 μm) for 10 min at room temperature. Then AdoMet (AdoMet) (25–150 μm) was added to initiate the reactions, which were incubated at 37 °C for 20 min. The data were fit to Equation 6 by non-linear regression, constraining Km = 17 μm and Vmax = 0.1128 nmol HMP/nmol ThiC/min.