FIGURE 3.

SANS analysis of FhuA, pb5, and complex. A, calculated and measured scattering curve of dFhuA solubilized in F₆-DigluM (triangles) at 46% D₂O. The 2FCP curve was calculated with the program Cryson, considering a 46% D₂O buffer and a 72% deuterated protein (solid line). χ² = 1.173 on the whole Q range. B, experimental scattering curves of isolated dFhuA solubilized in F₆-DigluM (triangles) or in complex with hpb5 (diamonds). Inset, Guinier analysis of the two data sets. C, scattering curves of dpb5 alone (crosses) or in complex with hFhuA solubilized in F₆-DigluM (squares) measured in 46% D₂O. Inset, Kratky representation of the dpb5 sample. D, pair distance distribution function, p(r), calculated using Gnom, of hFhuA-dpb5 (squares), dFhuA-hpb5 (diamonds), and dFhuA-dpb5 (circles) samples measured in a 46% D₂O buffer.