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. 2013 Jul 17;305(7):C716–C727. doi: 10.1152/ajpcell.00056.2013

Fig. 1.

Fig. 1.

SLC4A11 expression and localization. A: expression of the recombinant protein in HEK 293 cells transfected with phCMV2-HA-SLC4A11 at 24, 48, and 72 h. Detection was performed with anti-SLC4A11 antibody. SLC4A11 is detected as a 120-kDa band. B: average band density (n = 3). C: sulfo-NHS-S-S-Biotin (SNSB) Membrane preparation of SLC4A11-transfected cells. Detection was performed with anti-HA antibody. Total protein fraction (T), unbound protein fraction (U), and bound eluted membrane protein fraction (B). The presence of the protein in the eluted fraction indicates that the protein is localized in the membrane. D: immunohistochemistry using anti-HA in SLC4A11-transfected HEK293 shows membrane localization of the recombinant protein. E: immunohistochemistry using anti-HA in empty vector-transfected [control (Ctrl)] HEK293 cells. F: RT-PCR for pNBC and kNBC bicarbonate transporters in HEK293 cells and positive controls. G: RT-PCR for NHE1–5 in HEK293 cells.