Fig. 10.

Recovery from acid load in control and SLC4A11-transfected PS120 cells. The duration of the ammonium pulse was adjusted to provide more similar acidification in control and-transfected PS120 cells. A: alkalinization, acidification, and recovery from acid load in SLC4A11 or empty vector (control)-transfected PS120 cells following exposure and removal of NH₄Cl in BF Ringer in presence or absence of EIPA 10 μM. SLC4A11-transfected cells were acidified by incubation for 2 min with 10 mM NH₄Cl. To acidify control cells to the same extent, 4 min incubation with 20 mM NH₄Cl was required. Traces represent the average of 8 experiments. B: average ± SE of ΔpH_i of acidification after NH₄ removal. C: rate of rate of alkalinization recovery. *P < 0.05.