Fig. 2.
Effect of bicarbonate on pHi in SLC4A11-transfected HEK293. A: cells were loaded with BCECF-AM and pHi changes were analyzed in bicarbonate-free (BF) and bicarbonate-rich Ringer (BR). Traces represent the average of 12 experiments. Inset: pHi changes in bovine corneal endothelial cells (BCE). B: steady-state pHi in BF and BR conditions. C: delta pHi due to CO2-induced acidification (points 2–3 in A). D: initial maximum rate of alkalinization recovery was obtained by linear regression on the first 30 s of change. †P < 0.05, significantly different to paired BF condition. Labels 1–4 are explained on the text.