Fig. 5.

Recovery from acid load in SLC4A11-transfected cells. A: alkalinization, acidification, and recovery from acid load in SLC4A11 or empty vector (control)-transfected HEK293 cells following exposure (2 min) and removal of 10 mM NH₄Cl in BF Ringer. Traces represent the average of 7 experiments. B: NH₄⁺-induced acidification was calculated by linear regression of the first 20 s of pH change. C: delta pHi following NH₄⁺ removal. D: rate of alkalinization recovery was calculated by linear regression on the first 30 s of pH change corresponding to pH: 7.25 in BF. Rate of alkalinization recovery in presence of 5-(N-ethyl-N-isopropyl) amiloride (EIPA; 1 μM) was calculated by linear regression at matched pH: 7.25 and does not represent in this case the initial rate. *P < 0.05, significantly different from control. †P < 0.05, significantly different in the presence of EIPA. Labels 1–10 are explained in the text.