Fig. 3.

Effect of increased Hsc70 expression on whole cell ENaC and Hsp70 expression in MDCK cells. MDCK cells were treated with Dox (0–5 μg/ml) for 24 h, and expression of the ENaC subunits was determined by immunoblot analysis using antibodies directed to the epitope tags on each of the subunits. Hsp70 immunoreactivity was similarly determined, and GAPDH immunoreactivity was used as a loading control. Immunoblots are representative of 3 independent experiments.