Fig. 2.

With-no-lysine kinase 4 (WNK4) and the WNK4 Q562E mutant inhibit IBTX-sensitive whole cell currents in C-RIC cells. Experiments were performed with a whole cell patch mode, as described in materials and methods. The initial holding potential was −80 mV. Currents were measured with voltage steps from −80 mV to +100 mV (10-mV increments). A and B: representative recordings showing the effect of 50 nM IBTX on green fluorescent protein (GFP)-transfected C-RIC cells. C and D: representative recordings showing the effect of IBTX on WNK4 transfected C-RIC cells. E and F: representative recordings showing the effect of IBTX on WNK4 Q562E-transfected C-RIC cells. G: summary of the effects of WNK4 and WNK4 Q562E on IBTX-sensitive whole cell currents measured at +80 mV (n = 6 for GFP, n = 8 for WNK4, n = 6 for WNK4 Q562E; *P < 0.05 and ***P < 0.001, ANOVA followed by Tukey's multiple comparison test).