Table 3.
S-nitrosated or reversibly oxidized cysteine residues identified by mass shift labeling
| Sequence Id | Sequence Name | S-NO Peptide(s) | S-NO-Cysteine(s) |
|---|---|---|---|
| P02768 | Serum albumin | ALVLIAFAQYLQQC*PFEDHVKLVNEVTEFAK | C*58 |
| P21333 | Filamin-A | AEISC*TDNQDGTC*SVSYLPVLPGDYSILVK | C*1912, C*1920 |
| AHVVPC*FDASK | C*1157 | ||
| ALGALVDSC*APGLC*PDWDSWDASKPVTNAR | C*205, C*210 | ||
| ALGALVDSC*APGLC#PDWDSWDASKPVTNAR | C*205, C#210 | ||
| APLRVQVQDNEGC*PVEALVK | C*717 | ||
| APSVANVGSHC*DLSLKIPEISIQDMTAQVTSPSGK | C*2160 | ||
| ATC*APQHGAPGPGPADASK | C*2543 | ||
| C*APGVVGPAEADIDFDIIRNDNDTFTVK | C*810 | ||
| C*SGPGLSPGMVR | C*1453 | ||
| IVGPSGAAVPC*KVEPGLGADNSVVRFLPR | C*1018 | ||
| LQVEPAVDTSGVQC*YGPGIEGQGVFR | C*1260 | ||
| MDC*QEC*PEGYRVTYTPMAPGSYLISIK | C*2476, C*2479 | ||
| MDC#QEC*PEGYRVTYTPMAPGSYLISIK | C#2479, C*2479 | ||
| SPYTVTVGQAC*NPSAC*R | C*478, C*483 | ||
| SPYTVTVGQAC*NPSAC#RAVGRGLQPK | C*478, C#483 | ||
| P60709 | Actin, cytoplasmic 1 | SPYTVTVGQAC#NPSAC*R | C#478, C*483 |
| THEAEIVEGENHTYC*IR | C*2199 | ||
| VEYTPYEEGLHSVDVTYDGSPVPSSPFQVPVTEGC*DPSR | C*1353 | ||
| VGSAADIPINISETDLSLLTATVVPPSGREEPC*LLK | C*1997 | ||
| VQVQDNEGC*PVEALVKDNGNGTYSC#SYVPR | C*717 | ||
| VTYC*PTEPGNYIINIK | C*2107 | ||
| YWPQEAGEYAVHVLC*NSEDIR | C*649 | ||
| C*DVDIRKDLYANTVLSGGTTMYPGIADR | C*285 | ||
| FRC*PEALFQPSFLGMESC*GIHETTFNSIMK | C*257, C*272 | ||
| FRC#PEALFQPSFLGMESC*GIHETTFNSIMK | C#257, C*272 | ||
| FRC*PEALFQPSFLGMESC#GIHETTFNSIMK | C*257, C#272 | ||
| EKLC*YVALDFEQEMATAASSSSLEK | C*217 | ||
| P62736 | Actin, aortic smooth muscle | C*DIDIRKDLYANNVLSGGTTMYPGIADR | C*287 |
| C*PETLFQPSFIGMESAGIHETTYNSIMK | C*259 | ||
| EKLC*YVALDFENEMATAASSSSLEK | C*219 | ||
| P51911 | Calponin-1 | IGNNFMDGLKDGIILC*EFINKLQPGSVK | C*61 |
| RQIFEPGLGMEHC*DTLNVSLQMGSNKGASQR | C*238 | ||
| YC*LTPEYPELGEPAHNHHAHNYYNSA | C*273 | ||
| Q93052 | Lipoma-preferred partner | AYC#EPC#YINTLEQC*NVC*SKPIMER | C#465, C#468, C*476, C*480 |
| AYHPHC*FTC*VMC*HR | C*499, C*502, C*505 | ||
| AYHPHC#FTC*VMC*HR | C#499, C*502, C*505 | ||
| C*SVC*KEPIMPAPGQEETVR | C*536, C*539 | ||
| IVALDRDFHVHC*YR | C*566 | ||
| KTYITDPVSAPC*APPLQPK | C*364 | ||
| P68871 | Hemoglobin subunit-beta | SAQPSPHYMAAPSSGQIYGSGPQGYNTQPVPVSGQC*PPPSTR | C*262 |
| SLDGIPFTVDAGGLIHC*IEDFHKK | C*524 | ||
| VLGAFSDGLAHLDNLKGTFATLSELHC*DKLHVDPENFR | C*94 | ||
| LLGNVLVC*VLAHHFGKEFTPPVQAAYQK | C*113 | ||
| P14618 | Pyruvate kinase isozymes M1/M2 | AEGSDVANAVLDGADC*IMLSGETAKGDYPLEAVR | C*358 |
| AGKPVIC*ATQMLESMIK | C*326 | ||
| GIFPVLC*KDPVQEAWAEDVDLRVNFAMNVGK | C*474 | ||
| NTGIIC*TIGPASR | C*497 | ||
| P09382 | Galectin-1 | SFVLNLGKDSNNLC*LHFNPR | C*43 |
| FNAHGDANTIVC*NSKDGGAWGTEQR | C*61 | ||
| EAVFPFQPGSVAEVC*ITFDQANLTVK | C*89 | ||
| P13489 | Ribonuclease inhibitor | ELDLSNNC*LGDAGILQLVESVR | C*409 |
| TLPTLQELHLSDNLLGDAGLQLLC*EGLLDPQC*RLEK | C*134, C*142 | ||
| WAELLPLLQQC*QVVR | C*30 | ||
| P17661 | Desmin | QEMMEYRHQIQSYTC*EIDALKGTNDSLMR | C*333 |
| P04264 | Keratin, type II cytoskeletal 1 | MSGEC*APNVSVSVSTSHTTISGGGSR | C*497 |
| SGGGGGRFSSC*GGGGGSFGAGGGFGSR | C*49 | ||
| Q15746 | Myosin light chain kinase, smooth muscle | FDC*KIEGYPDPEVVWFKDDQSIR | C*1830 |
| HFQIDYDEDGNC*SLIISDVC*GDDDAKYTC*K | C*1865, C*1873, C*1882 | ||
| QAQVNLTVVDKPDPPAGTPC*ASDIR | C*1339 | ||
| Q9Y490 | Talin-1 | ALC*GFTEAAAQAAYLVGVSDPNSQAGQQGLVEPTQFAR | C*1434 |
| EC*ANGYLELLDHVLLTLQKPSPELK | C*2243 | ||
| TMQFEPSTMVYDAC*R | C*29 | ||
| P13645 | Keratin, type I cytoskeletal 10 | GSSGGGC*FGGSSGGYGGLGGFGGGSFR | C*66 |
| YC*VQLSQIQAQISALEEQLQQIR | C*401 | ||
| O14558 | Heat shock protein beta-6 | LFDQRFGEGLLEAELAALC*PTTLAPYYLR | C*46 |
| P68363 | Tubulin alpha-1B chain | AVC*MLSNTTAIAEAWAR | C*376 |
| AYHEQLSVAEITNAC*FEPANQMVK | C*295 | ||
| O00151 | PDZ and LIM domain protein 1 | AALANLC*IGDVITAIDGENTSNMTHLEAQNR | C*45 |
| IKGC*TDNLTLTVAR | C*73 | ||
| P69905 | Hemoglobin subunit-alpha | LLSHC*LLVTLAAHLPAEFTPAVHASLDKFLASVSTVLTSK | C*105 |
| P68104 | Elongation factor 1-alpha 1 | NMITGTSQADC*AVLIVAAGVGEFEAGISK | C*111 |
| SGDAAIVDMVPGKPMC*VESFSDYPPLGR | C*411 | ||
| Q5VTE0 | Putative elongation factor 1-alpha-like 3 | NMITGTSQADC*AVLIVAAGVGEFEAGISK | C*111 |
| SGDAAIVDMVPGKPMC*VESFSDYPPLGR | C*411 | ||
| P00338 | L-lactate dehydrogenase A chain | GLYGIKDDVFLSVPC*ILGQNGISDLVK | C*293 |
| LGVHPLSC*HGWVLGEHGDSSVPVWSGMNVAGVSLK | C*185 | ||
| Q53GG5 | PDZ and LIM domain protein 3 | AAAANLC*PGDVILAIDGFGTESMTHADAQDR | C*44 |
| P08779 | Keratin, type I cytoskeletal 16 | ISSVLAGGSC*RAPSTYGGGLSVSSR | C*40 |
| YC*MQLSQIQGLIGSVEEQLAQLR | C*369 | ||
| Q01518 | Adenylyl cyclase-associated protein 1 | ALLVTASQC*QQPAENKLSDLLAPISEQIK | C*93 |
| P67936 | Tropomyosin alpha-4 chain | EENVGLHQTLDQTLNELNC*I | C*247 |
| P62937 | Peptidyl-prolyl cis-trans isomerase A | HTGPGILSMANAGPNTNGSQFFIC*TAK | C*115 |
| IIPGFMC*QGGDFTR | C*62 | ||
| P04406 | Glyceraldehyde-3-phosphate dehydrogenase | IISNASC*TTNC#LAPLAK | C*152 |
| IISNASC#TTNC*LAPLAK | C*156 | ||
| P10768 | S-formylglutathione hydrolase | SVSAFAPIC*NPVLC*PWGK | C*176, C*181 |
| SYPGSQLDILIDQGKDDQFLLDGQLLPDNFIAAC*TEK | C*243 | ||
| P23142 | Fibulin-1 | RGYQLSDVDGVTC*EDIDEC*ALPTGGHIC*SYR | C*479, C*485, C*494 |
| C*LAFEC*PENYRR | C*551, C*556 | ||
| Q14315 | Filamin-C | FGGEHIPNSPFHVLAC*DPLPHEEEPSEVPQLR | C*1735 |
| SPFPVHVSEAC*NPNAC#R | C*472 | ||
| P02042 | Hemoglobin subunit-delta | GTFSQLSELHC*DKLHVDPENFR | C*113 |
| P06733 | Alpha-enolase | SGETEDTFIADLVVGLC*TGQIK | C*389 |
| Q9NZN4 | EH domain-containing protein 2 | FMC*AQLPNQVLESISIIDTPGILSGAK | C*138 |
| P21291 | Cysteine and glycine-rich protein 1 | GLESTTLADKDGEIYC*K | C*170 |
| NLDSTTVAVHGEEIYC*K | C*58 | ||
| P09211 | Glutathione S-transferase P | TLGLYGKDQQEAALVDMVNDGVEDLRC*K | C*102 |
| P07355 | Annexin A2 | EVKGDLENAFLNLVQC*IQNKPLYFADR | C*262 |
| P02787 | Serotransferrin | SAGWNIPIGLLYC*DLPEPR | C*156 |
| P07437 | Tubulin beta chain | LTTPTYGDLNHLVSATMSGVTTC*LR | C*239 |
| Q8WX93 | Palladin | RLLGADSATVFNIQEPEEETANQEYKVSSC*EQR | C*964 |
| P04075 | Fructose-bisphosphate aldolase A | ALSDHHIYLEGTLLKPNMVTPGHAC*TQK | C*240 |
| Q01995 | Transgelin | LVEWIIVQC*GPDVGRPDR | C*38 |
| P24844 | Myosin regulatory light polypeptide 9 | NAFAC*FDEEASGFIHEDHLR | C*109 |
| P35527 | Keratin, type I cytoskeletal 9 | YC*GQLQMIQEQISNLEAQITDVR | C*406 |
| P12277 | Creatine kinase B-type | SKDYEFMWNPHLGYILTC*PSNLGTGLR | C*283 |
| P48668 | Keratin, type II cytoskeletal 6C | ISIGGGSC*AISGGYGSR | C*77 |
| P08670 | Vimentin | QVQSLTC*EVDALKGTNESLER | C*328 |
| Q14195 | Dihydropyrimidinase-related protein 3 | AITIASQTNC*PLYVTK | C*248 |
| P54652 | Heat shock-related 70 kDa protein 2 | GPAIGIDLGTTYSC*VGVFQHGK | C*18 |
| Q99497 | Protein DJ-1 | GLIAAIC*AGPTALLAHEIGFGSK | C*106 |
| P60660 | Myosin light polypeptide 6 | ILYSQC*GDVMR | C*32 |
| P47756 | F-actin-capping protein subunit-beta | NLSDLIDLVPSLC*EDLLSSVDQPLK | C*36 |
| P02652 | Apolipoprotein A-II | EPC*VESLVSQYFQTVTDYGKDLMEK | C*29 |
| Q15942 | Zyxin | C*SVC*SEPIMPEPGRDETVR | C*504, C*507 |
| O00299 | Chloride intracellular channel protein 1 | EEFASTC*PDDEEIELAYEQVAK | C*223 |
| P01861 | Ig gamma-4 chain C region | WQEGNVFSC*SVMHEALHNHYTQK | C*305 |
Note some peptides were found in multiple states of S-nitrosation all of which were included in this table.
*
S-nitrosated cysteines;
#
non-S-nitrosated cysteines.