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TGF-β1 has no detectable effect on lactate dehydrogenase (LDH) release. PVD9902 cells were grown on 96-well plates for 3 days and then exposed to vehicle or TGF-β1 (5 ng/ml, 24 h). Cells in paired wells were lysed to determine maximum LDH activity. LDH activity in the apical medium is proportional to fluorescence at 590 nm. Results are summarized from 6 experiments.
