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TGF-β1 downregulates CFTR mRNA abundance. Expression of mRNA coding for CFTR in PVD9902 monolayers exposed to vehicle or TGF-β1 (5 ng/ml, 24 h) was determined by real-time RT-PCR. Data are summarized from 5 paired experiments and are expressed as the ΔCt between vehicle-treated cells and TGF-β1-treated cells for the reference 18S and for CFTR. *P < 0.05, significant difference.
