Fig. 8.

Role of Rap1A-Rho-ROCK signaling in filamin-2 Ser²¹¹³ phosphorylation in transiently transfected HEK293 cells. A: impact of expression vector (control) or constitutively activated Rap1A (Rap1A-CA) on the phosphorylation status of filamin-2 Ser²¹¹³ in the absence (solvent control) or presence of the Rho-associated kinase (ROCK) inhibitor fasudil (3 μM) determined by Western blot analysis using phospho-Ser²¹¹³-specific antibody. B: graphical representation of data. Data were normalized to total filamin and presented as means ± SE (n = 4–5). *P < 0.05.