Fig. 9.

The small GTPase Rap1A is necessary for phosphorylation of filamin-2 Ser²¹¹³ (p-filamin-2). Time course (hours, H) of p-filamin-2 induction in murine C57BL6 WT (Rap1A^+/+) or Rap1A-null (Rap1A^−/−) microVSM upon treatment with the adenylyl cyclase activator forskolin (FSK, 10 μM). A: Western blot analysis examining the phosphorylation status of filamin-2 Ser²¹¹³ using phospho-Ser²¹¹³-specific antibody (β-actin was used as a loading control). B: graphical representation of time course of p-filamin-2 by FSK. Data are presented as means ± SE (n = 4). Q, quiescent. *P < 0.05, **P < 0.001, ****P < 0.0001.