Fig. 8.

Gene expression is affected differently by positive WSSG and negative WSSG relative to WSS = 3.5 Pa and zero gradient. A: expression under positive WSSG (open bars) and negative WSSG (solid bars) was compared with baseline WSS (3.5 Pa) with WSSG = 0 for all 10 WSSG-sensitive genes that were also upregulated by high WSS. Note that expression under positive WSSG was significantly higher for 8 of the 10 genes, but expression in negative WSSG was not higher than baseline WSS for any of the 10 genes. B: same comparisons were made for the 22 WSSG-sensitive genes that were downregulated by high WSS. Expression under positive WSSG was lower compared with baseline WSS. In contrast, expression under negative WSSG was not significantly different from the baseline for all of the genes except GAS2 and FBXO32. Each bar represents the mean intensity ± SE from 3 flow experiments. *Expression difference relative to the baseline WSS of 3.5 Pa was ≥2-fold difference at 5% FDR.