Fig. 1.

Skeletal muscle-specific knockout of liver kinase B1 (LKB1). A: LKB1 protein content in resting gastrocnemius muscles from control (CON) and skeletal muscle-specific LKB1-knockout (KO) mice. B: LKB1 protein content in heart, liver, and kidney from CON and KO mice. C: representative immunoblots for LKB1 corresponding to data in A and C. LKB1 band is at ∼57 kDa. D: LKB1 activity in resting gastrocnemius samples from CON and KO mice, as determined by the rate of incorporation of phosphate from radiolabeled ATP into LKBtide. Data are presented as means ± SE; n = 6–12. ^aP < 0.05 vs. corresponding CON muscle. SOL, soleus; GAST, gastrocnemius, R QUAD, red quadriceps; W QUAD, white quadriceps.