Fig. 5.

PEPCK, cAMP, and phospho-cAMP response element-binding protein (CREB) levels are altered in the liver of Gcn2 KO mice. A: Western blotting of PEPCK protein in liver lysates from fed and 72-h-fasted Gcn2 KO and WT mice. Top, Western blot; bottom, PEPCK protein relative to tubulin (mean ± SE, n = 3; *P < 0.05 as indicated). B: cAMP level in livers of 24-h-fasted mice of indicated genotypes. cAMP levels are expressed as pmol/g of liver (mean ± SE, n = 4; *P < 0.05, Gcn2 KO vs. WT). C: CREB and phospho (p)-CREB protein levels in the liver of fed and 72-h-fasted Gcn2 KO and WT mice. Left, Western blot; right, ratio of phosphorylated CREB to total CREB protein (mean ± SE, n = 3; *P < 0.05 as indicated).