Table 5.
The effect of IMP on CER levels in cells treated with LPS, PA, or LPS + PA
| C16-CER | C18-CER | C20-CER | C22-CER | C24-CER | C24:1-CER | Total CER | |
|---|---|---|---|---|---|---|---|
| Control | 0.63 | 0.04 | 0.02 | 0.31 | 1.92 | 1.23 | 4.28 |
| Control+ IMP | 0.82 | 0.11 | 0.03 | 0.39 | 1.45 | 1.21 | 4.16 |
| LPS | 0.99 | 0.05 | 0.02 | 0.29 | 1.89 | 1.30 | 4.72 |
| LPS+IMP | 1.14 | 0.09 | 0.04 | 0.37 | 1.71 | 1.04 | 4.55 |
| PA | 1.30 | 0.24 | 0.14 | 1.32 | 1.70 | 1.13 | 6.00 |
| PA + IMP | 1.78 | 0.39 | 0.20 | 1.38 | 1.34 | 0.65 | 5.85 |
| LPS + PA | 4.15 | 1.05 | 0.56 | 3.05 | 2.89 | 2.33 | 14.57 |
| LPS + PA + IMP | 2.64 | 0.53 | 0.24 | 1.66 | 1.61 | 0.99 | 7.88 |
The unit of the values is pmol/nmol Pi. RAW 264.7 cells were treated with 1 ng/ml LPS, 100 μM PA, or both LPS and PA in the presence or absence of 50 μM IMP for 12 h. After treatment, cells were harvested and subjected to the lipidomic analysis of CERs, as described in materials and methods.