Table 1.
WT 6 mo LFD | KO 6 mo LFD | WT 6 mo HFD | KO 6 mo HFD | |
---|---|---|---|---|
TAG, mmol/l | 0.62 ± 0.06 | 0.65 ± 0.04 | 1.44 ± 0.14### | 1.25 ± 0.09### |
NEFA, mmol/l | 0.53 ± 0.03 | 0.74 ± 0.09 | 1.85 ± 0.14### | 1.58 ± 0.17### |
TNF-α, pg/ml | 13.5 ± 4.6 | 5.9 ± 1.3 | 22.5 ± 7.1 | 15.5 ± 6.2 |
IL-6, pg/ml | 17.6 ± 5.4 | 11.7 ± 4.5 | 34.3 ± 8.5 | 31.9 ± 9.1 |
Insulin, μg/l | 0.07 ± 0.02 | 0.27 ± 0.09 | 1.95 ± 0.3### | 2.87 ± 0.3*### |
Leptin, ng/ml | 13.34 ± 3.36 | 40.91 ± 13.36* | 53.23 ± 7.75### | 79.72 ± 5.72*## |
Adiponectin, ng/ml | 5,488 ± 678 | 4,902 ± 503 | 6,387 ± 503 | 5,296 ± 369 |
Values are means ± SE; n = 8 mice in each group. WT, wild type; LFD, low-fat diet; KO, knockout; TAG, triacylglycerol; NEFA, nonesterified fatty acid; TNF-α, tumor necrosis factor-α. Plasma was isolated from WT and IL-1 receptor I-deficient mice by cardiac puncture, and levels of metabolic markers were analyzed enzymatically (###P < 0.001, LFD vs. HFD; *P < 0.05, WT vs. KO).