Fig. 7.

Gadd45a contributes to HDAC4-mediated effects on skeletal muscle gene expression. A: C57BL/6 mice were subjected to unilateral sciatic nerve transection, and bilateral TA muscles were harvested 7 days later for qPCR analysis. mRNA levels in denervated muscles were normalized to levels in innervated muscles, which were set at 1. Data are means ± SE from 4–6 mice. *P ≤ 0.05. B: in C57BL/6 mice, one TA muscle was transfected with 20 μg p-HDAC4-FLAG, and the contralateral TA (Control) was transfected with 20 μg empty plasmid (pcDNA3). Bilateral TAs were harvested 7 days later for qPCR analysis. Data are means ± SE from 6 mice. *P ≤ 0.05. C: in C57BL/6 mice, one TA muscle was transfected with 20 μg p-Gadd45a-FLAG, and the contralateral TA (Control) was transfected with 20 μg empty plasmid (pcDNA3). Bilateral TAs were harvested 7 days later for qPCR analysis. Data are means ± SE from 4 mice. *P ≤ 0.05. D and E: TA muscles of C57BL/6 mice were transfected with 20 μg p-miR-Control, 20 μg p-miR-Gadd45a #1, or 20 μg p-miR-Gadd45a #2, as indicated, and then denervated 7 days later. Two days later, TAs were harvested for qPCR analysis. Data are means ± SE from 7–8 mice/condition. *P ≤ 0.05. F: TA muscles of C57BL/6 mice were transfected and harvested as in B and then used for qPCR analysis. Data are means ± SE from 6 mice. G: TA muscles of C57BL/6 mice were denervated and harvested as in A and then used for qPCR analysis. Data are means ± SE from 4–6 mice. *P ≤ 0.05.